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KMID : 1025520130550050427
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Journal of Animal Science and Technology
2013 Volume.55 No. 5 p.427 ~ p.434
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The Effect of Modified Cryopreservation Method on Viability of Frozen-thawed Primordial Germ Cell on the Korean Native Chicken (Ogye)
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Kim Hyun
Kim Dong-Hoon
Han Jae-Yong
Choi Seong-Bok
Ko Yeoung-Gyu
Do Yoon-Jung
Seong Hwan-Hoo
Kim Sung-Woo
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Abstract
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This study was conducted to establish methods for preserving chicken primordial germ cells (PGCs) for long-term storage in liquid nitrogen and for developmental engineering or preservation of species. The purpose of this study is to clarify the effects of fetal bovine serum (FBS) or chicken serum (CS) treatment on the viability of cryopreserved PGCs from Korean Native Chicken (Ogye). PGCs separated from a germinal gonad of an early embryo at day 5.5-6 (stage 28) were suspended in a freezing medium containing freezing and protective agents (dimethyl sulfoxide (DMSO), ethylene glycol (EG) and glycerol). The values from 0, 5, 10, and 15 % DMSO plus FBS treatment were 21.6, 30.36, 36.42, 50.39, and 48.36 %, respectively. The viability of PGCs after freeze-thawing was significantly higher for 10% EG plus FBS treatment than for 10% EG + FCS treatment (p<0.05) (64.36% vs. 50.66%). This study establishes a method for preserving chicken PGC that enables systematic storage and labeling of cryopreserved PGC in liquid nitrogen at a germplasm repository and an ease of entry into a database. In the future, the importance for this new technology is that poultry lines can be conserved while work is being conducted to improve the production of germline chimeras.
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KEYWORD
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Primordial germ cells (PGCs), EG, Viability, Korean native chicken (Ogye), Slow freezing
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